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KMID : 0380619930250010046
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Korean Journal of Food Science and Technology
1993 Volume.25 No. 1 p.46 ~ p.51
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Effect of Iron(Ⅱ) - ascorbate Complex on Protein and DNA of Phages
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Abstract
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The inactivating effect of iron(II)-ascorbate complex (Fe-Asc) on various phages was previously reported. This paper describes the molecular target in the phage virion attacked by Fe-Asc. The effect of Fe-Asc on protein was investigated with bovine serum albumin and the structural protein of phage Jl. There were no differences in the SDS-polyacrylamide gel electrophoresis (patterns of these two proteins when either they were treated) with Fe-Asc or not. Also, there were no changes in the amino acid composition and ultraviolet spectrum of the proteins. The effects of Fe-Asc on DNA was investigated with pUC18 DNA, M13mp8 DNA and λ DNA as well as DNA from phage Jl. Fe-Asc caused initially nicking of the supercoiled form of pUC18 DNA to yield the open circular form and then subsequently the linear form. Strand breaks were also confirmed with M13mp8 DNA and λDNA as well as Jl DNA. The results indicate that the strand breaks in phage DNA could be responsible for the inactivation of phages by Fe-Asc.
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